Serological analysis of varicella-zoster virus IgG antibody in healthy people aged 1～30 years in Liaoning Province / 中国生物制品学杂志

@#Abstract：Objective To carry out serological analysis of varicella⁃zoster virus（VZV）IgG antibody level in healthy people aged 1 ~ 30 years in Liaoning Province. Methods In October 2020，3～5 mL venous blood samples were collected from 617 healthy people aged 1～30 years selected from six counties and districts in Shenyang，Fuxin and Dandong of Liaoning Province by stratified random sampling method，of which serum samples were collected and determined for VZV IgG antibody level by ELISA. The positive rate of serum antibody and geometric mean concentration（GMC）of antibody were calculated and compared. Results Among 617 serum samples，302 samples were positive for VZV IgG antibody，the positive rate was 48. 947%，and the GMC was 112. 772 mIU／mL. The positive rate of VZV IgG antibody was 29. 670%～75. 789% and the GMC was 45. 508～366. 559 mIU／mL in healthy people of various ages. Both of the antibody positive rate（χ2 = 67. 104， P < 0. 001）and GMC（F = 20. 685，P < 0. 001）showed significant differences. The positive rates of VZV IgG antibody in male and female were 44. 817% and 53. 633% respectively，which showed significant difference（χ2 = 4. 779，P = 0. 029）， while the GMCs were 96. 983 and 133. 829 mIU／mL respectively（t = -1. 958，P = 0. 051）with no significant difference. The positive rates of VZV IgG antibody of healthy people in Shenyang，Fuxin and Dandong of Liaoning Province were 55. 224%，40. 201% and 51. 152% respectively with significant differences（χ2 = 9. 683，P = 0. 008），of which the positive rate of FuxinwassignificantlylowerthanthoseofShenyangandDandong（χ2 =9. 046and5. 013，P =0. 003and0. 025，respectively）； While the GMCs were 133. 523，85. 953 and 123. 713 mIU／mL respectively with no significant difference（F = 0. 514， P = 0. 598）. Among 617 serum samples，54 sampleswere suspicious，which remained within the criticalrange afterre⁃examina⁃ tion，while the gap between positive rate and the total percentage of positive and suspicious results gradually decreased with the increase of age，indicating that the immunity to varicella gradually increased with the increase of age. Conclusion The VZV⁃IgG antibody level of healthy people aged 1～30 years in Liaoning Province increased gradually with age，while the overall level was low. To control the spread of varicella virus，it is recommended to increase varicella vaccine coverage in vulnerable areas and susceptible population to build VZV immune barrier.

Effect of weight training on lower limb strength in martial arts athletes / Efeito do treinamento com pesos sobre a força dos membros inferiores em atletas de artes marciais / Efecto del entrenamiento con pesas en la fuerza de las extremidades inferiores en atletas de artes marciales

ABSTRACT Introduction: It is believed that weight training on the lower limbs in martial arts athletes can contribute to a better performance of the whiplash, a specific movement that encompasses pliometry, balance and strength. Objective: Study the effects of weight training on lower limb strength in martial arts athletes when performing the whiplash. Methods: A controlled experiment was conducted involving 100 martial arts athletes, randomly divided into two groups of characteristics without statically relevant differences. The experimental group received weight training, while the control group received no additional intervention to their daily training. Results: The vertical jump in the experimental group increased from 31.24 ± 6.65 cm to 36.00 ± 9.62 cm; the vertical jump in horizontal standing position increased from 195.49 ± 4.16 cm to 196.51 ± 7.49 cm; the approach vertical jump varied from 32.94 ± 9.21 cm to 37.60 ± 11. 50 cm; the jump from the local half squat position was from 34.55 ± 6.40 cm to 40.30 ± 8.14 cm; the maximum force of a squat with weights was from 140.81 ± 10.05 kg to 142.06 ± 10.23 kg; the maximum number of supine leg raises in one minute increased from 43.43 ± 6.15 to 48.05 ± 7.29. Conclusion: Weight training positively influenced lower limb strength in martial arts athletes, whiplash performance was elevated. Weight training is recommended in the daily training of martial arts athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: Acredita-se que o treinamento com pesos nos membros inferiores em atletas de artes marciais possa contribuir para um melhor desempenho do whiplash, um movimento específico que engloba pliometria, equilíbrio e força. Objetivo: Estudar os efeitos do treinamento com pesos sobre a força dos membros inferiores em aletas de artes marciais na execução do whiplash. Métodos: Foi conduzida uma experiência controlada envolvendo 100 atletas de artes marciais, divididos aleatoriamente em dois grupos de características sem diferenças estaticamente relevantes. O grupo experimental recebeu treinamento com pesos, enquanto o grupo de controle não recebeu nenhuma intervenção adicional ao treino cotidiano. Resultados: O salto vertical no grupo experimental elevou-se de 31,24 ± 6,65 cm para 36,00 ± 9,62 cm; o salto vertical em pé horizontal elevou-se de 195,49 ± 4,16 cm para 196,51 ± 7,49 cm; o salto vertical de aproximação variou de 32,94 ± 9,21 cm para 37,60 ± 11. 50 cm; o salto da posição de meio agachamento local foi de 34,55 ± 6,40 cm para 40,30 ± 8,14 cm; a força máxima de um agachamento com pesos foi de 140,81 ± 10,05 kg para 142,06 ± 10,23 kg; o número máximo de elevações de pernas supinas em um minuto elevou-se de 43,43 ± 6,15 para 48,05 ± 7,29. Conclusão: O treinamento com pesos influenciou positivamente na força dos membros inferiores em atletas de artes marciais, o desempenho do whiplash foi elevado. Recomenda-se o treinamento de pesos no treinamento diário dos atletas de artes marciais. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: Se cree que el entrenamiento con pesas sobre los miembros inferiores en atletas de artes marciales puede contribuir para una mejor ejecución del whiplash, un movimiento específico que engloba pliometría, equilibrio y fuerza. Objetivo: Estudiar los efectos del entrenamiento con pesas en la fuerza de los miembros inferiores en atletas de artes marciales al realizar el whiplash. Métodos: Se realizó un experimento controlado con 100 atletas de artes marciales, divididos aleatoriamente en dos grupos de características sin diferencias estadísticamente relevantes. El grupo experimental recibió entrenamiento con pesas, mientras que el grupo de control no recibió ninguna intervención adicional a su entrenamiento diario. Resultados: El salto vertical en el grupo experimental aumentó de 31,24 ± 6,65 cm a 36,00 ± 9,62 cm; el salto vertical horizontal de pie aumentó de 195,49 ± 4,16 cm a 196,51 ± 7,49 cm; el salto vertical de aproximación varió de 32,94 ± 9,21 cm a 37,60 ± 11. 50 cm; el salto desde la posición de media sentadilla local fue de 34,55 ± 6,40 cm a 40,30 ± 8,14 cm; la fuerza máxima de una sentadilla con pesas fue de 140,81 ± 10,05 kg a 142,06 ± 10,23 kg; el número máximo de elevaciones de piernas en decúbito supino en un minuto aumentó de 43,43 ± 6,15 a 48,05 ± 7,29. Conclusión: El entrenamiento con pesas influyó positivamente en la fuerza de las extremidades inferiores de los atletas de artes marciales y elevó el rendimiento de los whiplashs. Se recomienda el entrenamiento con pesas en el entrenamiento diario de los atletas de artes marciales. Nivel de evidencia II; Estudios terapéuticos-investigación de los resultados del tratamiento.

Role of human umbilical cord mesenchymal stem cells in the maturation and differentiation of dendritic cells in patients with severe acute pancreatitis and its mechanism in the inflammation modulation / 中华胰腺病杂志

Objective:To investigate the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the maturation and differentiation of dendritic cells (DCs) and the mechanism involved in the regulation of inflammation in patients with severe acute pancreatitis (SAP).Methods:The full-term fetal umbilical cords(about 4-5 cm) were collected from Zhengzhou Central Hospital Affiliated to Zhengzhou University after cesarean section. hUC-MSCs were isolated and cultured in primary culture. Flow cytometry was used for phenotype identification, adipogenic and osteogenic staining. 20 ml peripheral blood samples from 5 SAP patients were collected, and monocytes were isolated using lymphocyte separation solution and then induced by adding granulocyte macrophage colony-stimulating factor (GM-CSF), IL-4 and tumor necrosis factor (TNF)-α and cultured as DCs. According to different culture methods, DCs were divided into DCs group, hUC-MSCs+ DCs group and hUC-MSCs+ DCs+ NS398 group (NS398 was a specific inhibitor of COX-2, a downstream regulatory gene of NF-κB). The phenotype of DCs was detected by flow cytometry, and the levels of IL-1β, IL-lα, IL-2, IL-6 and IL-10 in the supernatant of cell culture for 24 hours were determined. The expression of toll like receptor (TLR)-4, IKKα and NF-κB-p65 were detected by Western blot.Results:The hUC-MSCs were successfully cultured, and their surface markers CD 90, CD 105 and CD 73 were positively expressed, and they could differentiate into adipocytes and bone cells. With the prolongation of culture time, DCs differentiated from immature to mature cells. Compared with the DCs group, the proportion of regulatory DCs (regDCs) was increased in the hUC-MSCs+ DCs group, and the marker CD 11b was significantly up regulated [(14.26±1.25)% vs (4. 87±0.58)%], CD 1a and CD 11c were significantly down regulated [(2.81±0.34)% vs (13.62±1.52)%, (3.88±0.5)% vs (11. 8±1.22)%]. All the difference were statistically significant ( P<0.05). The expression of IL-1β, INF-γ and IL-6 in culture supernatant were down regulated, but the difference was not statistically significant; The pro-inflammatory factor IL-1α was significantly decreased [(14.91±2.58)ng/L vs (30.19±7.75)ng/L], and the anti-inflammatory factor IL-10 was significantly increased [ (17.03±4.69)ng/L vs (1.83±0.14)ng/L]. The expression levels of NF-κB-p65 and TLR4 were significantly down regulated (0.74±0.02 vs 0.97±0.01, 0.89±0.01 vs 1.72±0.01), and the expression of IKKα protein was significantly up regulated (1.12±0.01 vs 0.21±0.01) in hUC-MSCs-DCs group. All the differences were statistically significant (all P value<0.05). Compared with DCs group and hUC-MSCs+ DCs group, the expression levels of NF-κB-p65 and TLR4 were significantly down regulated (0.34±0.01 vs 0.97±0.01, 0.74±0.02 and 0.14±0.01 vs 1.72±0.01, 0.89±0.01), while the expression of IKKα protein was significantly up regulated (1.68±0.01 vs 0.21±0.01, 1.12±0.01) in hUC-MSCs+ DCS+ NS398 group. All the differences were statistically significant (all P value<0.05). Conclusions:In SAP patients, hUC-MSCs can inhibit the maturation and differentiation of DCs, and induce CD 11bhigh CD 1alow CD 11clowrregDCs to participate in immune regulation, which may play an anti-inflammatory role by inhibiting the inflammatory cascade through TLR4/IKKα/NF-κB/COX-2 pathway.

CSCO ablation expert workshop report: Recommendations for the management of tumor ablation during the coronavirus disease 2019 epidemic

The coronavirus disease 2019 (COVID-19) has become a global pandemic since its outbreak in December 2019, which posed a threat to the safety and well-being of people on a global scale. Cancer patients are at high risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, and their critical morbidity and case fatality rates are high. The ablation expert committee of the Chinese Society of Clinical Oncology compiled corresponding expert recommendations. These recommendations summarize the preventive measures and management of tumor ablation treatment in medical institutions, including outpatient clinics, oncology wards, ablation operation room, and postablation follow-ups in accordance with the guidelines and protocols imposed by the National Health Commission of the People's Republic of China and the experience in management and prevention according to various hospitals. This consensus aims to reduce and prevent the spread of SARS-CoV-2 and its cross-infection between cancer patients in hospitals and provide regulatory advice and guidelines for medical personnel

Clinical significance of hypoxia-inducible factor 1α, and its correlation with p53 and vascular endothelial growth factor expression in resectable esophageal squamous cell carcinoma

Background: Hypoxia-inducible factor 1α (HIF-1α), p53, and vascular endothelial growth factor (VEGF) are important factors that facilitate tumor progression. The aims of our study were to investigate the expression of HIF-1α, p53, and VEGF in esophageal squamous cell carcinoma (ESCC) treated by curative surgery and to analyze their association with clinicopathological parameters and clinical outcome. Materials and Methods: The surgical specimens from 120 patients who had undergone potentially curative resection for ESCC were immunohistochemically assessed using monoclonal antibodies against HIF-1α, p53, and VEGF. Results: Positive rates of HIF-1α, p53, and VEGF expression were 61.7%, 56.7%, and 78.3%, respectively. No significant relationship was found between HIF-1α, p53, VEGF expression, and the analyzed clinicopathological parameters. There was no significant correlation between the expression of HIF-1α, p53, and VEGF. Univariate analysis revealed that overexpression of HIF-1α was associated with poor disease-free and overall survival (P = 0.023 and 0.01, respectively). Multivariate analysis demonstrated that upregulation of HIF-1α is an independent predictor for poor overall survival (P = 0.044). Conclusions: HIF-1α was a useful independent prognostic factor for surgically treated ESCC. Further studies with larger sample size are required to determine the relationship between the expression of HIF-1α, p53, VEGF, and clinicopathological parameters

A review of isolation methods, structure features and bioactivities of polysaccharides from Dendrobium species / 中国天然药物

Dendrobium, orchid, is a traditional Chinese herb medicine applied extensively as tonic and precious food for thou-sands of years recorded in ancient Chinese medical book "Shen Nong's Materia Medica". It's well known that bioactivities are usually related to the ingredients' basis. Based on the previous research, Dendrobium species contain amino acid, sesquiterpenoids, alkaloids and polysaccharides. As the bioactive substances, carbohydrate shows extensive activities in antitumor, antiglycation, immune-enhancing, antivirus, antioxidant, antitumor and etc. Therefore, as the main biologically active substance, the exact structures and latent activities of polysaccharides from Dendrobium species are widely focused on. In this review, we focus on the advancements of extraction methods and diversity of structures and bioactivities of polysaccharides obtained from Dendrobium species.

Effect of Stromal Cell Co-culture on Drug Resistance of Acute Myeloid Leukemia Cells and its Mechanism / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To study the effect of co-culture of stromal cells and acute myeloid leukemia(AML) cells on drug resistance of AML cells and its mechanism.</p><p><b>METHODS</b>Stromal cells were co-cultured with acute myeloid leukemia cell HL-60 and then were treated with DNR, HHT and Ara-C for observing the sensitivity of HL-60 cells to drugs after incubation with HS-5. At the same time, the the inhibitor LY294002 of PI3K/AKT signaling pathway was used to treat the cells, so as to explore whether the changes of HL-60 sensitivity is associated with the activation of PI3K/AKT signal pathway after co-culture of cells.</p><p><b>RESULTS</b>The statistical results of HL-60 cell inhibition rate showed that the HL-60 cell sensitivity to drugs was decreased after incubation with HS-5, the mRNA quantitation and immunblot detection showed that PI3K/AKT signaling pathway was activated after co-culture of HL-60 cells with HS-5 cells, in addition the CCND1, FOXO1, PTEN and other important genes were also changed significantly.</p><p><b>CONCLUSION</b>After co-culture of HL-60 cells with HS-5, some important molecules of PI3K/AKT signal pathway are changed, such as CCND1,FOXO1, PTEN, finally leading to the change of HL-60 cell sensitivity to drugs.</p>

Clinical analysis of 23 patients in hematologic neoplasms with fungemia / 白血病·淋巴瘤

Objective To investigate the epidemiology of patients in malignant hematologic neoplasms with fungemia and provide evidence for clinical therapy. Methods 23 cases of patients of malignant hematologic neoplasms with fungemia concurrent clinical material, the dangerous factor, the fungus colony classification as well as the treatment and the prognosis were carried on the review analysis. Results Each patient with fungemia was seriously ill with two or more predisposing factors;Candida ablicans accounted for 47%;the rate of candida parapsilosi, candida tropicalis were 17%, 12%. In the 23 cases of patients,13 cases of patients cure (56.5%), 8 cases of patients died (34.7%);2 cases of patients give up (8.6%). Conclusion Fungemia in the patients of malignant hematologic neoplasms usually occurred with predisposing factors. Important measures to reduce mortality include curing the underlying diseases, emphasis on mornitoring fungal pathogen and susceptibility tests, early diagnosis and compliance medication principles.

The efficacy of high-dose cytarabine for patients with t(8;21) AML and with normal karyotype AML / 中华血液学杂志

<p><b>OBJECTIVE</b>To compare the efficacy of high-dose cytarabine (HD-Ara-C) based chemotherapy for post-remission treatment in patients with t(8;21) (q22;q22) AML-M2 and those with normal karyotype AML-M2.</p><p><b>METHODS</b>AML-M2 patients were grouped into with (21 cases) or without (23 cases) t(8;21) (q22;q22) karyotype groups. After achieved remission by induction therapy, all patients received four cycles of HD-Ara-C (3 mg/m2 per 12 hours by three-hour infusion day 1 to day 3) with either mitoxantrone (7 mg m(-2) d(-1)) or aclarubicin (30 mg m(-2) d(-1)) or etoposide (70 mg m(-2) d(-1)) for 3d as post-remission treatment.</p><p><b>RESULTS</b>Relapse rate in the t(8;21) and the normal karyotype groups was 29% and 57% respectively (P<0.05); 3 year disease-free survival (DFS) rate was 71% and 43% respectively (P < 0.05). and 3 year over-all survival (OS) rate was 76% and 65% respectively (P >0.05).</p><p><b>CONCLUSION</b>Four cycles of high-dose cytarabine based combination chemotherapy as post-remission treatment improves long-term disease-free survival in patients with t(8;21) (q22;q22) AML-M2.</p>

Application of all-trans retinoic acid combining chemotherapy and As4S4 in the maintenance treatment of patients with acute promyelocytic leukemia / 中华血液学杂志

<p><b>OBJECTIVE</b>To compare the efficacy of all-trans retinoic acid (ATRA) combining chemotherapy and As4S4 with ATRA combining chemotherapy for the maintenance treatment of patients with acute promyelocytic leukemia (APL).</p><p><b>METHODS</b>Sixty patients with APL induced to complete remission by ATRA and consolidated by chemotherapy were randomly divided into two groups. Thirty patients as As4S4 group received ATRA + As4S4 + chemotherapy, and another thirty patients as non-As4S4 group were treated only with ATRA + chemotherapy as maintenance therapy. The therapeutic effects, side effects and PML-RARalpha gene expression were analyzed.</p><p><b>RESULTS</b>The three-year continuous complete remission (CCR) rate was 90.0% for As4S4 group and 61.1% for non-As4S4 group, the difference being statistically significant. Significant difference was also found in the positive rate of PML-RARalpha fusion gene between the two groups. The side effects were mild.</p><p><b>CONCLUSION</b>APL patients in maintenance therapy with ATRA + 6-MP + MTX + As4S4 can obtain a higher CCR.</p>

Progress in drug therapy for retinoblastoma / 眼科

Retinoblastoma is the most common intraoeular malignant tumor in children.In its treatment,drug therapy is one of the most effective and irreplaceable methods.To reduce the toxicity of drugs,enhance the therapeutic effect and lessen drug resistance, some new drug deliver systems and new medicines for retinoblastoma are coming into being.Ophthalmic arterial infusion,fibrin sealant and iontophoresis are newly-found drug deliver systems.And the latest drugs under research include nutlins,phenoxazine derivative Phx-1,combretastatin A4 phosphate,2-deoxy-d-glucose and histone deacetylase inhibitors,etc.The following text is focused on the two aspects of the drug therapy for retinoblastoma.

A 20-year prospective study on risk factors for myocardial infarction of 5,137 men in Capital Steel and Iron Company / 中华预防医学杂志

<p><b>OBJECTIVE</b>To identify independent risk factors for myocardial infarction (MI) in Chinese men and to develop a model to predict risk profile of an individual suffering MI.</p><p><b>METHODS</b>Study sample included 5 137 men aged 45.2 +/- 7.8 years who came from a cohort in Beijing Capital Steel and Iron Company, based on the three surveys on coronary heart disease conducted in 1974, 1979 and 1980, respectively. Demographic data and other risk factors, such as life style, medical history, blood pressure, total serum cholesterol level (TC), etc. were collected according to the same protocol in 1980. All the participants were followed up for MI in an average period of 20.84 years until 2001.</p><p><b>RESULTS</b>(1) There were 122 cases with MI identified during the period of follow-up, with an incidence of MI 117.4 per 100 000 person-years. Age of more than 50, smoking, higher systolic and diastolic blood pressure (SBP and DBP) levels, higher TC all were identified as important risk factors of MI. (2) Incidence of MI increased with TC. An increment of 0.52 mmol/L of TC significantly increased relative risk of MI by approximately 40% after adjusted for age, blood pressure and smoking. (3) An increment of 20 mm Hg in SBP or 10 mm Hg in DBP associated with a 40% increase in incidence of MI, adjusting for age, TC and smoking. (4) Smoking was the most risky factors for MI. Smokers had 2.3 times risk of MI, after as compared to non-smokers (or its incidence increased by 137%), after adjusting for blood pressure, TC and age, etc. (5) Incidence of MI increased by 20% with increment of five-year of age in those aged over 50 (P < 0.05), after adjusting for blood pressure, TC and smoking. And, (6) finally, based on multivariate logistic and Cox regression analyses, a model containing several risk factors, such as age, blood pressure, TC and smoking, was developed to predict individual's risk for afflicting MI.</p><p><b>CONCLUSIONS</b>Results of this prospective study showed several established risk factors for MI, including age, blood pressure, TC and smoking all as independent predictors of MI in Chinese men. It is clear and rational that intervention and modification of those traditional risk factors can lead to a decrease in coronary events in Chinese population.</p>